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Microsatellite loci development for three catfish species from northwestern South America Neotropical Ichthyology
Restrepo-Escobar,Natalia; Márquez,Edna J..
ABSTRACT The Neotropical catfish species Ageneiosus pardalis, Pimelodus grosskopfii, and Sorubim cuspicaudus are important fishery resources in Colombia that show historical declines in their capture. This study used next-generation sequencing with 454 FLX technology (Roche Applied Science) and bioinformatics analysis to develop between 18 and 24 microsatellite loci for these species. The novel microsatellite loci showed high values of polymorphic information content -PIC (A. pardalis: 0.601-0.903, P. grosskopfii: 0.748-0.946 and S. cuspicaudus: 0.383-0.876), and the average number of alleles/locus ranged from 7-15 for A. pardalis, 9-30 for P. grosskopfii and 5-14 for S. cuspicaudus. The average observed and expected heterozygosities were respectively,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fish; Molecular markers; Next-generation sequencing; Siluriformes.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000100210
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Population genetics of three threatened catfish species in heterogeneous environments of the Cauca River, Colombia Neotropical Ichthyology
Restrepo-Escobar,Natalia; Yepes-Acevedo,Anny Johanna; Márquez,Edna Judith.
ABSTRACT Neotropical catfishes Ageneiosus pardalis, Pimelodus grosskopfii and Sorubim cuspicaudus are migratory fishes of commercial importance that exhibit decreasing populations due to overfishing and other anthropic interventions. This study used species-specific microsatellite loci to test the hypothesis that threatened fish populations show genetic vulnerability signs and are genetically structured in the middle and lower sections of the Cauca River. The studied species exhibit genetic diversity levels higher than the average values reported for Neotropical Siluriformes; however, they seem to have suffered recent bottlenecks and they present significant endogamy levels that are higher for the critically endangered catfish P. grosskopfii. Furthermore,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fish; Genetic diversity; Genetic structure; Microsatellites; Siluriformes.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100204
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